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Gold nanoparticles on titanium and interaction with prototype protein

Identifieur interne : 005268 ( Main/Exploration ); précédent : 005267; suivant : 005269

Gold nanoparticles on titanium and interaction with prototype protein

Auteurs : J. Daniel Padmos [Canada] ; Paul Duchesne ; Michael Dunbar [Canada] ; Peng Zhang [Canada]

Source :

Journal of Biomedical Materials Research Part A [ 1549-3296 ] ; 2010-10.

RBID : ISTEX:0A13BF5982F85FBB30162534DE9367E024AAFAB1

Descripteurs français

Wicri :
- topic : Oxyde, Titane.

English descriptors

Abstract

Modifying titanium (Ti) implant surfaces with functional proteins can strengthen the interface between prosthesis and bone. A prototype system was developed using gold nanoparticles (AuNPs) to immobilize proteins onto Ti. An electroless (galvanic displacement) deposition method was first used to form AuNPs of controlled size and coverage on commercial Ti foil (giving Ti‐AuNPs). Parameters were then modified to create two groups of discs (n = 26) with different average AuNP diameters. Scanning electron microscopy and X‐ray photoelectron spectroscopy were used to characterize the morphology and surface structure of Ti‐AuNPs. To study the interaction of Ti‐AuNPs with proteins, Ti discs (n = 8) modified with plain AuNPs and discs (n = 8) modified with thiol (HSRCOOH)‐functionalized AuNPs were treated with lysozyme solution. The amount and activity of the lysozyme on the discs were examined with Micro‐BCA and enzymatic assays. Lysozyme was immobilized onto the discs, and the assays showed that the discs with thiol‐functionalized AuNPs, discs with bare AuNPs, and Ti controls had average lysozyme adsorptions of 23 × 104, 2.3 × 104, and 5.7 × 104 μg/m2, respectively. The activity assays showed that 21.5, 18.4, and 12.5% of the adsorbed lysozyme was active on the discs with thiol‐functionalized AuNPs, discs with bare AuNPs, and Ti controls, respectively. This technique holds promise for binding functional biomolecules to surgical implants, hence possibly creating implant surfaces that react to their local environment. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.

Url:

https://api.istex.fr/document/0A13BF5982F85FBB30162534DE9367E024AAFAB1/fulltext/pdf

DOI: 10.1002/jbm.a.32826

Affiliations:

Canada

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Le document en format XML

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<term>Anova test</term>
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<term>Dalhousie university</term>
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<term>Lysozyme coverage</term>
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<term>Metallic gold</term>
<term>Micrococcus lysodeikticus</term>
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<term>Normality tests</term>
<term>Nova scotia</term>
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<term>Protein solution</term>
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<term>Different groups</term>
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<term>Enzymatic activity</term>
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<term>Etching</term>
<term>Gold nanoparticles</term>
<term>Gold surfaces</term>
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<front><div type="abstract" xml:lang="en">Modifying titanium (Ti) implant surfaces with functional proteins can strengthen the interface between prosthesis and bone. A prototype system was developed using gold nanoparticles (AuNPs) to immobilize proteins onto Ti. An electroless (galvanic displacement) deposition method was first used to form AuNPs of controlled size and coverage on commercial Ti foil (giving Ti‐AuNPs). Parameters were then modified to create two groups of discs (n = 26) with different average AuNP diameters. Scanning electron microscopy and X‐ray photoelectron spectroscopy were used to characterize the morphology and surface structure of Ti‐AuNPs. To study the interaction of Ti‐AuNPs with proteins, Ti discs (n = 8) modified with plain AuNPs and discs (n = 8) modified with thiol (HSRCOOH)‐functionalized AuNPs were treated with lysozyme solution. The amount and activity of the lysozyme on the discs were examined with Micro‐BCA and enzymatic assays. Lysozyme was immobilized onto the discs, and the assays showed that the discs with thiol‐functionalized AuNPs, discs with bare AuNPs, and Ti controls had average lysozyme adsorptions of 23 × 104, 2.3 × 104, and 5.7 × 104 μg/m2, respectively. The activity assays showed that 21.5, 18.4, and 12.5% of the adsorbed lysozyme was active on the discs with thiol‐functionalized AuNPs, discs with bare AuNPs, and Ti controls, respectively. This technique holds promise for binding functional biomolecules to surgical implants, hence possibly creating implant surfaces that react to their local environment. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.</div>
</front>
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Gold nanoparticles on titanium and interaction with prototype protein

Gold nanoparticles on titanium and interaction with prototype protein

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English descriptors

Abstract

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